This is an exciting book reflects the current area of international human health concern. Genome editing technologies have demonstrated a plethora of benefits to the biological sciences. CRISPR- Cas9, a beneficial gene editing tool, has become a robust strategy for making alterations to the genome of organisms and a potent weapon in the treatment of Covid-19 and cancer tumor. It has revealed an excellent clinical potential for cancer therapy by discovering novel targets and has provided the researchers with the perception about how tumors respond to drug therapy. The CRISPR-Cas9 system is a genome-editing tool that has had a significant impact on biological research in recent years, and also shows promise for the treatment of patients with genetic disorders, Covid-19, cancers and cardiovascular disease and so forth.